mouse monoclonal anti cdx2 antibody (Biogenex)
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Biogenex
mouse monoclonal anti cdx2 antibody
Mouse Monoclonal Anti Cdx2 Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti-cdx2/anti+cdx2+mouse/pm42098360-284-16-21
Average 86 stars, based on 1 article reviews
Mouse Monoclonal Anti Cdx2 Antibody, supplied by Biogenex, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+monoclonal+anti-cdx2/anti+cdx2+mouse/pm42098360-284-16-21
Average 86 stars, based on 1 article reviews
mouse monoclonal anti cdx2 antibody - by Bioz Stars,
2026-09
86/100 stars
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Blocking Assay:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Incubation:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Immunofluorescence:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the In Utero:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Generated:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Derivative Assay:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Over Expression:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Comparison:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Expressing:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Fluorescence:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Labeling:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Marker:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the In Vitro:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Staining:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Control:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the TUNEL Assay:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Nucleic Acid Electrophoresis:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Transfection:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Real-time Polymerase Chain Reaction:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Western Blot:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the Reverse Transcription Polymerase Chain Reaction:Article Title: Luteolin supplementation during porcine oocyte maturation improves the developmental competence of parthenogenetic activation and cloned embryos Article Snippet: For CDX2 staining, blastocysts were blocked with 10% normal goat serum for an additional 45 min, then incubated at 4 °C overnight with cytochrome c (Abcam, Cambridge, UK), cleaved caspase 3 (Cell Signaling Technology, Danvers, MA, USA) or Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for immunofluorescence: mouse monoclonal anti-Oct3/4 (clone C-10) (Santa Cruz, SC-5279) 1:100; rabbit polyclonal anti-Oct3/4 (clone H-134) (Santa Cruz, SC-9081) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit monoclonal anti-CK7 (Abcam, ab181598) 1:200; rabbit monoclonal anti-CK7 (Abcam, ab68459) 1:200; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; rabbit monoclonal anti-syndecan1 (Abcam, ab128936) 1:400; Article Title: Lysophosphatidic acid and sphingosine-1-phosphate are apical polarity cues in multiple organoid systems. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-Acetyl-tubulin (1:1000) Sigma Cat#T6793; RRID: AB_477585 Mouse monoclonal anti-Arl13B (1:200) NeuroMab Cat#N295B/66; RRID: AB_2877361 Mouse monoclonal anti-β-Catenin (1:300) BD Biosciences Cat#610153; RRID: AB_397554 Rabbit polyclonal anti-Cingulin (1:1000) Invitrogen Cat#PA5-55661; RRID: AB_2639733 Rabbit monoclonal anti-Cleaved Caspase-3 (1:1000) BD Biosciences Cat#559565; RRID: AB_397274 Mouse monoclonal anti-CDX2 (1:500) Article Title: The Wnt/TCF7L1 transcriptional repressor axis drives primitive endoderm formation by antagonizing naive and formative pluripotency Article Snippet: Rat monoclonal anti-Nanog eBioscienceTM Cat#14-5761-80; RRID:AB_763613 Goat polyclonal anti-Gata6 R&D systems Cat#AF1700, RRID:AB_2108901 Mouse monoclonal anti-Gata4 BD Biosciences Cat#560327, RRID:AB_1645188 Mouse monoclonal anti-Cdx2 Biogenex Cat#AM392GP Rabbit monoclonal anti-(active)β-catenin Cell Signaling Technology Cat#8814, RRID:AB_11127203 Goat polyclonal anti-Tcf3 (M-20) (Tcf7l1) Santa Cruz Biotechnology Cat#sc-8635, RRID:AB_2199133 Goat polyclonal anti-Nestin Santa Cruz Biotechnology Cat#sc-21248, RRID: AB_2148925 Mouse monoclonal anti-βcatenin BD Biosciences Cat#610154, RRID:AB_397555 Rabbit monoclonal anti-Tcf1 (C63D9) Cell Signaling Technology Cat#2203, RRID:AB_2199302 Rabbit monoclonal anti-Lef1 (C12A5) Cell Signaling Technology Cat#2230, RRID:AB_823558 Article Title: Mouse embryo model derived exclusively from embryonic stem cells undergoes neurulation and heart development. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A10042; RRID: AB_2534017 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 568 Thermo Fisher Scientific Cat# A-11057; RRID: AB_2534104 Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31571; RRID: AB_162542 Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-31573; RRID: AB_2536183 Donkey anti-Goat IgG (H+L) CrossAdsorbed Secondary Antibody, Alexa Fluor 647 Thermo Fisher Scientific Cat# A-21447; RRID: AB_2535864 Donkey Anti-Rat IgG H&L (Alexa Fluor 647) preadsorbed antibody Abcam Cat# ab150155; RRID: AB_2813835 Bacterial and virus strains 5-alpha competent E. coli New England Biolabs C2987I Chemicals, peptides, and recombinant proteins b-estradiol Sigma-Aldrich Cat# E8875-1G CHIR99021 Cambridge Stem Cell Institute N/A Doxycycline Sigma-Aldrich Cat# D9891-5G Fgf2 Cambridge Stem Cell Institute N/A Recombinant Mouse FGF-4 (aa 67-202) R&D Systems Cat# 7486-F4-025 Heparin Sigma-Aldrich Cat# H3149-25KU Insulin-transferrin-selenium-ethanolamine ThermoFisher Scientific Cat# 51,500-056 Leukemia inhibitory factor Cambridge Stem Cell Institute N/A N-acetyl-L-cysteine Sigma-Aldrich Cat# A7250 PD0325901 Cambridge Stem Cell Institute N/A Progesterone Sigma-Aldrich Cat# P8783-1G Y-27632 STEMCELL Technologies Cat# 72,304 Critical commercial assays Gateway BP Clonase II Enzyme mix Invitrogen Cat# 11,789-100 Gateway LR Clonase II Enzyme mix Invitrogen Cat# 11,791-100 Lipofectamine 3000 Transfection Reagent Invitrogen Cat# L3000001 RNeasy Mini Kit Qiagen Cat# 74,104 SYBR Green PCR Master Mix Applied Biosystems Cat# 4,368,708 Deposited data Single-cell RNA sequencing data This manuscript ArrayExpress: E-MTAB-12140 Code for analyizing single-cell RNA sequencing data This manuscript https://doi.org/10.5281/zenodo.7021607 Experimental models: Cell lines Mouse: CAG-GFP ESCs Rhee et al. (2006) N/A Mouse: CAG-GFP/tetO-mCherry/tetOCdx2 ESCs This manuscript N/A Mouse: CAG-GFP/tetO-mCherry/tetOGata4 ESCs Amadei et al., 2021 N/A Mouse: Cerl-GFP/tetO-Gata4 ESCs Amadei et al., 2021 N/A (Continued on next page) e2 Cell Stem Cell 29, 1445–1458.e1–e8, October 6, 2022 Article Title: Modified-Chitosan/siRNA Nanoparticles Downregulate Cellular CDX2 Expression and Cross the Gastric Mucus Barrier Article Snippet: Total protein extracts (30–50 μg) were subjected to standard sodium dodecyl sulphate–polyacrylamide gel electrophoresis, transferred onto a nitrocellulose membrane (Amersham, GE Healthcare, UK) and incubated with primary antibodies overnight at 4°C: Article Title: Complete human day 14 post-implantation embryo models from naive ES cells Article Snippet: The following antibodies and dilutions were used for cell immunofluorescence: rabbit monoclonal anti-BST2/Tetherin antibody (EPR20202-150) (Abcam, ab243230) 1:100; goat polyclonal anti-Sox17 (R&D, AF1924) 1:100; rabbit polyclonal anti-Gata4 (Abcam, ab84593) 1:100; goat polyclonal anti-FoxF1 (R&D, AF4798) 1:100; goat polyclonal anti-Gata3 (R&D, AF2605) 1:100; Article Title: Spindle shape and volume differ in high- and low-quality metaphase II oocytes Article Snippet: In brief: Optical coherence microscopy non-invasively visualizes metaphase II spindles allowing for quantitative analysis of their volume and shape, which may prove useful in the assessment of the oocyte quality.. Using a mouse model, we showed also that analysis of spindle length combined with morphokinetics improves the evaluation of the |
